천연추출물의 발모촉진 및 탈모 예방에 미치는 영향

This study examined the effects of natural extracts including black bean, Ginkgo biloba L. fruits, and green tea, on hair growth and scalp improvement. For the research method, black beans, dried ginkgo biloba, and green tea leaves were immersed in 10 times distilled water at a ratio of 2 : 1 : 1, extracted with hot water at 100 ° C for 2 hours, and Eclipse Plus C18 (4.6 × 250 mm, 5 μm) was used. As the mobile phase, 70% methanol diluted with ultrapure water was used. The antibacterial activity of the extract was evaluated using Malassezia furfur, and the antioxidant activity was evaluated by measuring the electron donating ability to DPPH. For proliferative activity of human dermal papilla cells and fibroblasts, 3×104 (100 μl/well) of cells were aliquoted in a 96 well plate and cultured for 24 hours, followed by MTT assay. After administration of natural extracts by concentration, and after incubation, DMSO solution was added, and after 30 minutes extraction on a plate shaker, absorbance was measured using ELISA at 570 nm. Antimicrobial activity of natural extracts on Pityrosporum ovale and Malassezia furfur were investigated. In antioxidative activities of natural extracts, DPPH radical scavenging activity was about 62.40±4.8 % at 5.0 mg/mL. On the other hand, cell proliferation in NIH3T3 cells and HFDPC cells were determined. Natural extracts promoted the proliferation of NIH3T3 cells, a human epidermal cells by about 10 % at 0.02 % concentration. HFDPC cells, a hair follicle dermal papilla cells were significantly stimulated in cell proliferation by above 40% at 0.10% concentration. These results suggest that natural extracts have antimicrobial activity, antioxidant effects, induce cell proliferation, and could be used for hair loss prevention and scalp improvement.