Cloning and Analysis of Medium-Chain-Length Poly(3-Hydroxyalkanoate)Depolymerase Gene of Pseudomonas luteola M13-4
Cloning and Analysis of Medium-Chain-Length Poly(3-Hydroxyalkanoate)Depolymerase Gene of Pseudomonas luteola M13-4
박인재(주식회사 휴럼(오창)); YOUNG HA RHEE(Chungnam National University); NAM-YOUNG CHO(Daejeon University); KWANG-SOO SHIN(Daejeon University)
16권 12호, 1935~1939쪽
초록
The gene encoding the extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA) depolymeraseof Pseudomonas luteola M13-4, phaZPlu, was cloned andanalyzed. It was found to be 849 bp, with a deduced proteinof 282 amino acids, and was revealed to have a typical leaderPlurevealed relatively low identity (69 to 72%) with those ofother Pseudomonas MCL-PHA depolymerases. In comparisonwith the amino acid sequences of all available MCL-PHAdepolymerases, the depolymerase was found to consist ofthre domains in sequential order; signal peptide, an N-terminal substrate binding domain, and a catalytic domain,indicating that PhaZPlu belongs to the type IV depolymerasesamino acid.
Abstract
The gene encoding the extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA) depolymeraseof Pseudomonas luteola M13-4, phaZPlu, was cloned andanalyzed. It was found to be 849 bp, with a deduced proteinof 282 amino acids, and was revealed to have a typical leaderPlurevealed relatively low identity (69 to 72%) with those ofother Pseudomonas MCL-PHA depolymerases. In comparisonwith the amino acid sequences of all available MCL-PHAdepolymerases, the depolymerase was found to consist ofthre domains in sequential order; signal peptide, an N-terminal substrate binding domain, and a catalytic domain,indicating that PhaZPlu belongs to the type IV depolymerasesamino acid.
- 발행기관:
- 한국미생물·생명공학회
- 분류:
- 생물학