A Histone Deacetylase Inhibitor, Trichostatin A, Enhances adiosensitivity by Abrogating G2/M Arrest in Human Carcinoma Cells
A Histone Deacetylase Inhibitor, Trichostatin A, Enhances adiosensitivity by Abrogating G2/M Arrest in Human Carcinoma Cells
In Ah Kim(서울대학교); 김진호(서울대학교); Jin Hee Shin(서울대학교); Il Han Kim(서울대학교); 김재성(서울대학교); Hong-Gyun Wu(서울대학교); Eui Kyu Chie(서울대학교); Yong Ho Kim(Soonchunhyang University); Bo-Kyung Kim(Dankook University); Semie Hong(Konkuk University); Seok Won Park(Chung-Ang University); Sung Whan Ha(서울대학교); Charn Il Park(서울대학교)
37권 2호, 122~128쪽
초록
Purpose: Histone deacetylase inhibitors (HDIs) are emerging as potentially useful components in anticancer therapy. In this study, we tried to confirm the radiosensitizing effect of richostatin A (TSA) on a panel of uman carcinoma cell lines and elucidate its mechanism of interaction. Materials and Methods: A549, HeLa and Caski cells were exposed to TSA for 18 hr prior to irradiation, and the cell survival then measured using a clonogenic assay. Western blot and flow cytometric analyses, for histone acetylation, and cell cycle and apoptosis, respectively,were also performed. Results: TSA increased the acetylation of histone H3. The pretreatment of TSA consistently radiosensitized all three cell lines. The SF2 (surviving fraction at 2 Gy) of TSA-treated cells was significantly lower than that of mock treated cells. The SER (sensitizer enhancement ratio) increased in all 3 cell lines, in concentration dependent manners. The TSA treated cells showed abrogation of radiation-induced G2/M arrest, in a concentration dependent manner. Conclusion: The pretreatment of TSA enhanced the radiosensitivity of a panel of human carcinoma cells, which was attributed, in part, to the abrogation of radiationinduced G2/M arrest.
Abstract
Purpose: Histone deacetylase inhibitors (HDIs) are emerging as potentially useful components in anticancer therapy. In this study, we tried to confirm the radiosensitizing effect of richostatin A (TSA) on a panel of uman carcinoma cell lines and elucidate its mechanism of interaction. Materials and Methods: A549, HeLa and Caski cells were exposed to TSA for 18 hr prior to irradiation, and the cell survival then measured using a clonogenic assay. Western blot and flow cytometric analyses, for histone acetylation, and cell cycle and apoptosis, respectively,were also performed. Results: TSA increased the acetylation of histone H3. The pretreatment of TSA consistently radiosensitized all three cell lines. The SF2 (surviving fraction at 2 Gy) of TSA-treated cells was significantly lower than that of mock treated cells. The SER (sensitizer enhancement ratio) increased in all 3 cell lines, in concentration dependent manners. The TSA treated cells showed abrogation of radiation-induced G2/M arrest, in a concentration dependent manner. Conclusion: The pretreatment of TSA enhanced the radiosensitivity of a panel of human carcinoma cells, which was attributed, in part, to the abrogation of radiationinduced G2/M arrest.
- 발행기관:
- 대한암학회
- 분류:
- 의학일반