반응표면분석법에 의한 메밀(Fagopyrum esculentum M.) 새싹 기능성분의 추출 조건 최적화
Optimization of Extraction Conditions to Obtain Functional Components from Buckwheat (Fagopyrum esculentum M.) Sprouts, using Response Surface Methodology
박기재(한국식품연구원); 김범근(한국식품연구원); 정진웅(한국식품연구원); 김종찬(한국식품연구원); 이명헌(한림성심대학교); 조영심(한국식품연구원); 정희용(한국식품연구원); 임정호(한국식품연구원)
16권 5호, 734~741쪽
초록
본 실험은 메밀새싹의 추출조건에 따른 수율, 총 페놀, α-glucosidase 저해활성능, 및 ACE 저해활성능에 대해 반응표면 분석법을 이용하여 추출조건을 최적화하였다. 중심합성계획에 따라 추출온도(0∼100 ℃), 추출시간(0∼12 hr) 및 에탄올 농도(0∼100%)를 달리하였을 때 반응 표면 회귀식의 R2는 수율, 총 페놀, α-glucosidase 저해활성능 및 ACE 저해활성능에서 각각 0.9461(p<0.001), 0.8875(p<0.005), 0.9186(p<0.005) 및 0.8667(p<0.005)로 나타내었으며, 추출조건별 총 페놀 함량, α-glucosidase 저해활성능 및 ACE 저해활성능에 대한 반응표면을 superimposing하여 얻은 최적 추출조건 범위는 추출온도 0∼70℃, 추출시간 2∼8 hr 및 에탄올 농도 30∼80%로 나타내었다. 최적 추출조건 범위내의 임의의 조건인 추출온도 15℃, 추출시간 5 hr 및 에탄올 농도 50 %를 회귀식에 대입하여 얻은 예측값은 수율 15.18%, 총 페놀 함량 189.27 mg/100 g, α-glucosidase 저해활성능 77.44 % 및 ACE 저해활성능 85.24%으로 예측되었으며, 실제 실험을 통해 얻어진 값은 수율 16.18%, 총 페놀 함량 175.57 mg/100 g, α-glucosidase 저해활성능 79.17% 및 ACE 저해활성능 81.60%으로 매우 유사하게 나타났다.
Abstract
Response surface methodology (RSM) was used to optimize extraction conditions for functional components of buckwheat (Fagopyrum esculentum). A central composite design was applied to investigate the effects of three independent variables, namelyextraction temperature (X1), extraction time (X2), and ethanol concentration (X3), on responses including extraction yield (Y1), total phenolic content in the extract (Y2), α-glucosidase inhibition activity (Y3), and acetylcholine esterase (ACE) inhibition activity (Y4). Data were analyzed using an expert design strategy and statistical software. The maximum yield was 24.95% (w/w) at 55.75℃ extraction temperature, 8.75 hextraction time, and 15.65% (v/v) ethanol. The maximum total phenolic yield was 222.45 mg/100 g under the conditions of 28.11℃ extraction temperature, 8.65 h extraction time, and 81.72% (v/v) ethanol. The maximum α-glucosidase inhibition activity was 85.38% at 9.62℃, 7.86 h, and 57.58% (v/v) ethanol. The maximum ACE inhibition activity was 86.91% under extraction conditions of 10.12℃, 4.86 h, and 44.44% (v/v) ethanol. Based on superimposition of a four-dimensional RSM with respect to levels of total phenolics, α-glucosidase inhibition activity, and ACE inhibition activity, obtained under various extraction conditions, the optimum ranges of conditions were an extraction temperature of 0-70℃, an extraction time of 2-8 h, and an ethanol concentration of 30-80% (v/v).
- 발행기관:
- 한국식품저장유통학회
- 분류:
- 식품과학