Two pHZ1358 Derivative Vectors for Efficient Gene Knockout in Streptomyces
Two pHZ1358 Derivative Vectors for Efficient Gene Knockout in Streptomyces
He, Yunlong(Shanghai Jiaotong University); Zhijun Wang(Shanghai Jiaotong University); Linquan Bai(Shanghai Jiao Tong University); Jingdan Liang(Shanghai Jiaotong University); Xiufen Zhou(Shanghai Jiaotong University); Zixin Deng(Shanghai Jiao Tong University)
20권 4호, 678~682쪽
초록
The deletion of sti from the Streptomyces plasmid pIJ101made its derivative pHZ1358 an efficient vector for gene disruption and replacement. Here, pHZ1358 was further optimized by the construction of a derivative plasmid pJTU1278, in which a cassette carrying multiple cloning sites and a lacZ selection marker were introduced for convenient plasmid construction in E. coli. In addition,the oriT region of pJTU1278 was also deleted, generating a vector (pJTU1289) that can be used specifically for PCR-targeting. The efficient usage of these vectors was demonstrated by the deletion of the gene involved in avermectin biosynthesis in S. avermitilis.
Abstract
The deletion of sti from the Streptomyces plasmid pIJ101made its derivative pHZ1358 an efficient vector for gene disruption and replacement. Here, pHZ1358 was further optimized by the construction of a derivative plasmid pJTU1278, in which a cassette carrying multiple cloning sites and a lacZ selection marker were introduced for convenient plasmid construction in E. coli. In addition,the oriT region of pJTU1278 was also deleted, generating a vector (pJTU1289) that can be used specifically for PCR-targeting. The efficient usage of these vectors was demonstrated by the deletion of the gene involved in avermectin biosynthesis in S. avermitilis.
- 발행기관:
- 한국미생물·생명공학회
- 분류:
- 생물학