국내 가축 유래 대장균에서 CTX-M 및 TEM형 extended-spectrum β-lactamases의 검출
Detection of CTX-M and TEM type extended-spectrum β-lactamases in Escherichia coli isolated from livestocks in Korea
조재근(대구광역시 보건환경연구원); 김기석(경북대학교); 성명숙(경상북도 가축위생시험소); 김진현(경북대학교)
34권 1호, 37~43쪽
초록
This study was conducted to investigate the prevalence and genotypes of extended-spectrum β-lactamase (ESBL) in 377 Escherichia coli isolated from healthy and sick animals. Two isolates (0.5%), each of which were isolated from diseased swine and chicken, respectively, were confirmed as ESBL producing isolates by double disk synergy test, and showed a multidrug resistant phenotype. Minimum inhibitory concentration of cefotaxime for the two ESBL producing isolates were 3∼4 times higher than those of ceftazidime, respectively. By PCR and sequencing, one isolate from swine have both bla_CTX-15 and bla_TEM-1, and one isolate from chicken have bla_CTX-15 and bla_TEM-116. Also, these genes were transferred to E. coli J53 by conjugation. These two isolates showed unrelated pulsed-field gel electrophoresis. To our knowledge, this is the first time that bla_TEM-116 gene was identified in E. coli isolated from animals in Korea. These results suggest more prudent use of third- generation cephalosporins, and surveillance and monitoring for ESBL producing E. coli in both animals and their environments should be necessary.
Abstract
This study was conducted to investigate the prevalence and genotypes of extended-spectrum β-lactamase (ESBL) in 377 Escherichia coli isolated from healthy and sick animals. Two isolates (0.5%), each of which were isolated from diseased swine and chicken, respectively, were confirmed as ESBL producing isolates by double disk synergy test, and showed a multidrug resistant phenotype. Minimum inhibitory concentration of cefotaxime for the two ESBL producing isolates were 3∼4 times higher than those of ceftazidime, respectively. By PCR and sequencing, one isolate from swine have both bla_CTX-15 and bla_TEM-1, and one isolate from chicken have bla_CTX-15 and bla_TEM-116. Also, these genes were transferred to E. coli J53 by conjugation. These two isolates showed unrelated pulsed-field gel electrophoresis. To our knowledge, this is the first time that bla_TEM-116 gene was identified in E. coli isolated from animals in Korea. These results suggest more prudent use of third- generation cephalosporins, and surveillance and monitoring for ESBL producing E. coli in both animals and their environments should be necessary.
- 발행기관:
- 한국동물위생학회
- 분류:
- 수의학