Association of a Pyruvate Kinase M2(PKM2) Polymorphism with Back Fat Thickness in Berkshire Pigs
Association of a Pyruvate Kinase M2(PKM2) Polymorphism with Back Fat Thickness in Berkshire Pigs
조은석(Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA); 전현정(Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA); 이시우(Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA); 박종운(Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA); Sebastian Raveendar(Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA); 장길원(농촌진흥청 국립축산과학원(수원)); 김태헌(농촌진흥청 국립축산과학원(수원)); 이경태(농촌진흥청 국립축산과학원(수원))
55권 6호, 515~520쪽
초록
Pyruvate kinase M2(PKM2) is a key regulatory enzyme in the glycolytic pathway. It is one of four pyruvate kinase isoenzymes that widely differ in their occurrence according to tissue type. PKM2 is expressed in differentiated tissues, such as fat tissues, lung, as well as normal proliferating cells, embryonic cells, and tumor cells. The objective of this study was to investigate the association of single nucleotide polymorphisms(SNPs) in the PKM2 gene with meat quality traits in Berkshire pigs. We detected a SNP(g.34341 A>G) in the 3’UTR region of the PKM2 gene in 670 Berkshire pigs through DNA sequencing. Three genotypes, AA, AG, and GG, were found for this SNP, but based on an association analysis with meat quality traits, genotype AA was significantly associated with thicker back fat than genotype GG(p=0.027). Therefore, the g.34341 A>G polymorphism in the 3’UTR region of the porcine PKM2 gene could be applied in pig breeding programs to improve back fat thickness.
Abstract
Pyruvate kinase M2(PKM2) is a key regulatory enzyme in the glycolytic pathway. It is one of four pyruvate kinase isoenzymes that widely differ in their occurrence according to tissue type. PKM2 is expressed in differentiated tissues, such as fat tissues, lung, as well as normal proliferating cells, embryonic cells, and tumor cells. The objective of this study was to investigate the association of single nucleotide polymorphisms(SNPs) in the PKM2 gene with meat quality traits in Berkshire pigs. We detected a SNP(g.34341 A>G) in the 3’UTR region of the PKM2 gene in 670 Berkshire pigs through DNA sequencing. Three genotypes, AA, AG, and GG, were found for this SNP, but based on an association analysis with meat quality traits, genotype AA was significantly associated with thicker back fat than genotype GG(p=0.027). Therefore, the g.34341 A>G polymorphism in the 3’UTR region of the porcine PKM2 gene could be applied in pig breeding programs to improve back fat thickness.
- 발행기관:
- 한국축산학회
- 분류:
- 축산학