Genetic Diversity of Farmers’Preferred Sorghum Accessions and Improved Lines from ICRISAT Reveal a Disconnect Between Innovation and Technology Transfer
Genetic Diversity of Farmers’Preferred Sorghum Accessions and Improved Lines from ICRISAT Reveal a Disconnect Between Innovation and Technology Transfer
Muigai Paul Kimani(Egerton University); James Otieno Owuoche(Egerton University); Francis Nyamu Wachira(Association for strengthening Agricultural Research in Eastern and Central Africa); Esther Kimani(Kenya Agricultural Research Institute Biotechnology Centre); Erick Kimutai Cheruiyot(Egerton University, Department of Crops)
17권 2호, 97~102쪽
초록
The genetic diversity of 65 accessions of sorghum [Sorghum bicolor (L.) Moench] collected from various farmers and germplasmlines from ICRISAT-Kenya were analyzed. Simple sequence repeats (SSR) markers were used in order to determine the extent anddistribution of its genetic diversity. Twenty-nine (29) SSRs markers were polymorphic and a total of 192 alleles were detected whichshowed diversity. The number of alleles per primer ranged from 2 to 17, with an average of 6.62. The range of polymorphism informationcontent (PIC) ranged from 0.03 to 0.86, with total average of 0.82. According to the results analyzed, estimates of the meanallelic pattern across the two populations was generated; expected heterozygosity (He; 0.45, 0.54), average observed alleles (Na;3.40, 6.20), number of private allele (0.23, 3.03), and Shannon information index (I; 0.85, 1.13) for farmer and ICRISAT-Kenyagermplasm, respectively. The expected heterozygosity (He) varied from 0 to 0.26 with an average of 0.05. The Neighbor-joiningphenogram based on Nei’s genetic distance grouped the 65 accessions into three main groups. The analysis of molecular variance(AMOVA) revealed that 99% of the total genetic variation was within accessions in a population whereas the genetic variationamong populations in accessions accounted for 1% of the total genetic variation. Genetic diversity in ICRISAT sorghum materialcompared to the farmer’s collection suggested little infiltration of improved germplasm to the farmers
Abstract
The genetic diversity of 65 accessions of sorghum [Sorghum bicolor (L.) Moench] collected from various farmers and germplasmlines from ICRISAT-Kenya were analyzed. Simple sequence repeats (SSR) markers were used in order to determine the extent anddistribution of its genetic diversity. Twenty-nine (29) SSRs markers were polymorphic and a total of 192 alleles were detected whichshowed diversity. The number of alleles per primer ranged from 2 to 17, with an average of 6.62. The range of polymorphism informationcontent (PIC) ranged from 0.03 to 0.86, with total average of 0.82. According to the results analyzed, estimates of the meanallelic pattern across the two populations was generated; expected heterozygosity (He; 0.45, 0.54), average observed alleles (Na;3.40, 6.20), number of private allele (0.23, 3.03), and Shannon information index (I; 0.85, 1.13) for farmer and ICRISAT-Kenyagermplasm, respectively. The expected heterozygosity (He) varied from 0 to 0.26 with an average of 0.05. The Neighbor-joiningphenogram based on Nei’s genetic distance grouped the 65 accessions into three main groups. The analysis of molecular variance(AMOVA) revealed that 99% of the total genetic variation was within accessions in a population whereas the genetic variationamong populations in accessions accounted for 1% of the total genetic variation. Genetic diversity in ICRISAT sorghum materialcompared to the farmer’s collection suggested little infiltration of improved germplasm to the farmers
- 발행기관:
- 한국작물학회
- 분류:
- 농학