GC-MS를 이용한 사람 뇨시료 중 비만치료제 분석 및 비만치료제 남용 현황의 법과학적 고찰
Development of a Simultaneous Detection and Quantification Method of Anorectics in Human Urine Using GC-MS and its Application to Legal Cases
최혜영(국립과학수사연구원); 이재신(국립과학수사연구원); 장문희(국립과학수사연구원); 양원경(국립과학수사연구원); 김은미(국립과학수사연구원); 최화경(국립과학수사연구원)
57권 6호, 420~425쪽
초록
Phentermine (PT) and phenmetrazine (PM) have been widely used as anti-obesity drugs. These drugs shouldbe used with caution due to its close relation to amphetamine in its structure and toxicity. PT and PM, amphetamine-typeanorectics, have recently been considered as alternatives for methamphetamine abuse in Korea. In addition, the misuse andabuse of PT and PM obtained by illegal sources such as the internet become a serious social problem. In the present study,a simultaneous detection and quantification method for determining PT and PM in human urine was developed and validatedaccording to the international guidelines. The urine samples were screened using a fluorescence polarization immunooassayand analyzed by gas chromatography mass spectrometry (GC-MS) after extraction using automatic solid phase extraction(SPE) with a mixed-mode cation exchange cartridge and derivatization with pentafluoropropionic anhydride (PFPA). Thevalidation results for selectivity, linearity, limits of detection (LOD) and quantification (LOQ), intra- and inter-assay precisionand accuracy and recovery were satisfactory. The validated method was successfully applied to authentic urine samples collectedfrom 38 drug abuse suspects. PT and/or PM were identified with or without methamphetamine in urine samples. Abuse of PT and PM have increased continuously in Korea, therefore, closer supervision of the inappropriate use of anoreticsis necessary.
Abstract
Phentermine (PT) and phenmetrazine (PM) have been widely used as anti-obesity drugs. These drugs shouldbe used with caution due to its close relation to amphetamine in its structure and toxicity. PT and PM, amphetamine-typeanorectics, have recently been considered as alternatives for methamphetamine abuse in Korea. In addition, the misuse andabuse of PT and PM obtained by illegal sources such as the internet become a serious social problem. In the present study,a simultaneous detection and quantification method for determining PT and PM in human urine was developed and validatedaccording to the international guidelines. The urine samples were screened using a fluorescence polarization immunooassayand analyzed by gas chromatography mass spectrometry (GC-MS) after extraction using automatic solid phase extraction(SPE) with a mixed-mode cation exchange cartridge and derivatization with pentafluoropropionic anhydride (PFPA). Thevalidation results for selectivity, linearity, limits of detection (LOD) and quantification (LOQ), intra- and inter-assay precisionand accuracy and recovery were satisfactory. The validated method was successfully applied to authentic urine samples collectedfrom 38 drug abuse suspects. PT and/or PM were identified with or without methamphetamine in urine samples. Abuse of PT and PM have increased continuously in Korea, therefore, closer supervision of the inappropriate use of anoreticsis necessary.
- 발행기관:
- 대한약학회
- 분류:
- 약학