A new phenanthrene derivative and two diarylheptanoids from the roots of Brassica rapa ssp. campestris inhibit the growth of cancer cell lines and LDL-oxidation
A new phenanthrene derivative and two diarylheptanoids from the roots of Brassica rapa ssp. campestris inhibit the growth of cancer cell lines and LDL-oxidation
Qian Wu(KyungHee University); 백남인(경희대학교); Jin-Gyeong Cho(Kyung Hee University); 유기현(경희대학교); 정태숙(한국생명공학연구원); 박지해(경희대학교); Su-Yeon Kim(Kyung Hee University); Ji-Hyun Kang(KRIBB); 정인식(경희대학교); 최명숙(경북대학교); 이경태(경희대학교); Hae-Gon Chung(GangHwa Agricultural R&D Center, Korea); 방면호(경희대학교)
36권 4호, 423~429쪽
초록
Brassica rapa ssp. campestris (Brassicaceae) isa conical, deep purple, edible root vegetable commonlyknown as a turnip. We initiated phytochemical and pharmacologicalstudies to search for biological active compoundsfrom the roots of B. rapa ssp. campestris. Weisolated a novel phenanthrene derivative, 6-methoxy-1-[10-methoxy-7-(3-methylbut-2-enyl)phenanthren-3-yl]undecane-2,4-dione, named brassicaphenanthrene A (3) alongwith two known diarylheptanoid compounds, 6-paradol (1)and trans-6-shogaol (2), through the repeated silica gel(SiO2), octadecyl silica gel, and Sephadex LH-20 columnchromatography. The chemical structures of the compoundswere determined by spectroscopic data analyses includingnuclear magnetic resonance, mass spectrometry, ultravioletspectroscopy, and infra-red spectroscopy. All compoundsexhibited high inhibitory activity against the growth ofhuman cancer lines, HCT-116, MCF-7, and HeLa, with IC50values ranging from 15.0 to 35.0 lM and against LDL-oxidationwith IC50 values ranging from 2.9 to 7.1 lM.
Abstract
Brassica rapa ssp. campestris (Brassicaceae) isa conical, deep purple, edible root vegetable commonlyknown as a turnip. We initiated phytochemical and pharmacologicalstudies to search for biological active compoundsfrom the roots of B. rapa ssp. campestris. Weisolated a novel phenanthrene derivative, 6-methoxy-1-[10-methoxy-7-(3-methylbut-2-enyl)phenanthren-3-yl]undecane-2,4-dione, named brassicaphenanthrene A (3) alongwith two known diarylheptanoid compounds, 6-paradol (1)and trans-6-shogaol (2), through the repeated silica gel(SiO2), octadecyl silica gel, and Sephadex LH-20 columnchromatography. The chemical structures of the compoundswere determined by spectroscopic data analyses includingnuclear magnetic resonance, mass spectrometry, ultravioletspectroscopy, and infra-red spectroscopy. All compoundsexhibited high inhibitory activity against the growth ofhuman cancer lines, HCT-116, MCF-7, and HeLa, with IC50values ranging from 15.0 to 35.0 lM and against LDL-oxidationwith IC50 values ranging from 2.9 to 7.1 lM.
- 발행기관:
- 대한약학회
- 분류:
- 약학