계피 유도체 2'-benzoyloxycinnamaldehyde가 경구편평상피암종YD-10B 세포의 세포사멸과 자가포식 유도
Cinnamaldehyde Derivative, 2'-benzoyloxycinnamaldehyde (BCA), Induces Apoptosis and Autophagy in Human Oral Squamous Carcinoma YD-10B Cells
김현우(조선대학교); 김수아(동국대학교); 권성민(원광대학교 치과대학 대전치과병원 구강병리과); 윤정훈(원광대학교); 이주용(조선대학교); 안상건(조선대학교 치과대학 구강병리학교실)
38권 5호, 247~256쪽
초록
Cinnamaldehyde is known to have the antitumor effects in vitro and in vivo. In this study, we showed a potent and irreversiblecytotoxic activity of the Cinnamaldehyde derivative 2'-benzoyloxycinnamaldehyde (BCA) in human Squamous oral cell carcinoma cell,YD-10B. BCA induced YD-10B cell apoptosis in a dose-responsive manner. BCA-induced apoptosis was associated with correspondingincreases in a series of key components in the mitochondria-mediated apoptosis pathways, followed by caspase cleavage and PARPactivation. We also observed that BCA induced autophagy through Akt/mTOR pathway in YD-10B cells. BCA treatment increased LC3B-IIexpression, and induced the formation of autophagosomes and autophagic vacuoles. These experimental findings suggest that BCAis a potent inhibitor of cell proliferation in YD-10B cells and provide new insights about leading to the possible development of anew therapeutic agent.
Abstract
Cinnamaldehyde is known to have the antitumor effects in vitro and in vivo. In this study, we showed a potent and irreversiblecytotoxic activity of the Cinnamaldehyde derivative 2'-benzoyloxycinnamaldehyde (BCA) in human Squamous oral cell carcinoma cell,YD-10B. BCA induced YD-10B cell apoptosis in a dose-responsive manner. BCA-induced apoptosis was associated with correspondingincreases in a series of key components in the mitochondria-mediated apoptosis pathways, followed by caspase cleavage and PARPactivation. We also observed that BCA induced autophagy through Akt/mTOR pathway in YD-10B cells. BCA treatment increased LC3B-IIexpression, and induced the formation of autophagosomes and autophagic vacuoles. These experimental findings suggest that BCAis a potent inhibitor of cell proliferation in YD-10B cells and provide new insights about leading to the possible development of anew therapeutic agent.
- 발행기관:
- 대한구강악안면병리학회
- 분류:
- 치의학