Kjeldahl 증류법을 활 용한 질산성-질소 및 암 모니아성-질소 안정동위원소비 분석 및 질소오염원 추적 사례 연구
Introduction of Kjeldahl Digestion Method for Nitrogen Stable Isotope Analysis (δ15N-NO3 and δ15NNH4) and Case Study for Tracing Nitrogen Source
김민섭(국립환경과학원); 박태진(국립환경과학원 물환경공학연구과); 윤슥희(국립환경과학원); 임보라(국립환경과학원); 신경훈(한양대학교); 권오상(국립환경과학원); 이원석(국립환경연구원)
48권 3호, 147~152쪽
초록
Nitrogen (N) loading from domestic, agricultural and industrial sources can lead to excessive growth of macrophytes or phytoplankton in aquatic environment. Many studies have used nitrogen stable isotope ratios to identify anthropogenic nitrogen in aquatic systems as a useful method for studying nitrogen cycle. In this study to evaluate the precision and accuracy of Kjeldahl processes, two reference materials (IAEA-NO-3, N-1) were analyzed repeatedly. Measured the δ15N-NO3 and δ15N-NH4 values of IAEA-NO-3 and IAEA-N-1 were 4.7±0.2‰ and 0.4±0.3‰, respectively, which are within recommended values of analytical uncertainties. Also, we investigated spatial patterns of δ15N-NO3 and δ15N-NH4 in effluent plumes from a waste water treatment plant in Han River, Korea. δ15N-NO3 and δ15N-NH4 values are enriched at downstream areas of water treatment plant suggesting that dissolved nitrogen in effluent plumes should be one of the main N sources in those areas. The current study clarifies the reliability of Kjeldahl analytical method and the usefulness of stable isotopic techniques to trace the contamination source of dissolved nitrogen such as nitrate and ammonia.
Abstract
Nitrogen (N) loading from domestic, agricultural and industrial sources can lead to excessive growth of macrophytes or phytoplankton in aquatic environment. Many studies have used nitrogen stable isotope ratios to identify anthropogenic nitrogen in aquatic systems as a useful method for studying nitrogen cycle. In this study to evaluate the precision and accuracy of Kjeldahl processes, two reference materials (IAEA-NO-3, N-1) were analyzed repeatedly. Measured the δ15N-NO3 and δ15N-NH4 values of IAEA-NO-3 and IAEA-N-1 were 4.7±0.2‰ and 0.4±0.3‰, respectively, which are within recommended values of analytical uncertainties. Also, we investigated spatial patterns of δ15N-NO3 and δ15N-NH4 in effluent plumes from a waste water treatment plant in Han River, Korea. δ15N-NO3 and δ15N-NH4 values are enriched at downstream areas of water treatment plant suggesting that dissolved nitrogen in effluent plumes should be one of the main N sources in those areas. The current study clarifies the reliability of Kjeldahl analytical method and the usefulness of stable isotopic techniques to trace the contamination source of dissolved nitrogen such as nitrate and ammonia.
- 발행기관:
- 한국수생태학회
- 분류:
- 환경공학