A novel M2e-multiple antigenic peptide providing heterologous protection in mice
A novel M2e-multiple antigenic peptide providing heterologous protection in mice
Feng Wen(Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences); Ji-Hong Ma(Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences); Hai Yu(Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences); Fu-Ru Yang(Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences); Meng Huang(Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences); Yan-Jun Zhou(Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences); Ze-Jun Li(Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences); Xiu-Hui Wang(Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences); Guo-Xin Li(Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences); Yi-Feng Jiang(Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences); Wu Tong(Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences); Guangzhi Tong(Chinese Academy of Agricultural Sciences)
17권 1호, 71~78쪽
초록
Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
Abstract
Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
- 발행기관:
- 대한수의학회
- 분류:
- 수의학