Escherichia coli가 생성하 는 CTXᅳM형 Extended-Spectrum β-lactamase
Escherichia coli Producing CTX-M Extended-Spectrum βᅳLactamase
황현용(고신대학교); 박재선(고신대학교); 배일권(고신대학교 의과대학 진단검사의학교실); 이유내(고신대학교); 정석훈(고신대학교 의과대학)
22권 1호, 58~67쪽
초록
Background β -lactam antibiotics have been broadly used to treat gram negative microorganisms, which resulted in the occurrence of antibiotics-resistant microorganisms. Microorganisms mainly achieve resistance to antibiotics by producing β-lactamases that decrease activities of antibiotics. Various types of CTX-M extended-spectrum β -lactamases (ESBLs) have been known since CTX-M-1 and CTX-M-2 ESBLs were reported in 1996. CTX-M-14 β-lactamases were reported in Seoul and Cheju Island of Korea in 2001. Until now, there has been no CTX-M type ESBL report in Korea. So, the research on the identification of the CTX-M type ESBLs and their genetic types using E. coli resistant to or intermediate to cefotaxime were performed. Methods Total of 365 isolates of Escherichia coli from Kosin University Hospital in Busan for a six-month periods were examined. Disk diffusion test for antibiotics susceptibility, double disk synergy test for confirming ESBL, polymerase chain reaction (PCR) for identifying genetic types of CTX-M ESBLs, minimal inhibitory concentration tests of CTX-M ESBLs and isoelectricfocusing (IEF) for measuring the pi of β一lactamase were tested using E. coli resistant or intermediate to cefotaxime. Results Thirty one out of 365 E. coli isolates were resistant to or intermediate to cefotaxime. Fifteen out of 31 E. coli isolates resistant or intermediate to cefotaxime were positive to double disk synergy, 6 isolates of which were positive to PCR by CTX-M-2F and CTX-M-2R primers. MICs of cefotaxime to isolates carrying CTX-M ESBL were as high as ≥128 mg/L. MICs of ceftazidime were relatively low as 2-64 mg/L. Isoelectric point was 8.4 for blacCTX-M-3 and 8.6 for blaCTX-M-i5, respectively. Conclusion CTX-M-3 /? -lactamase and CTX-M-15 P β-lactamase found in this study are genetically different from previous types of CTX—M—type ESBLs in Korea. Also newly found CTX-M type ESBLs are expected to cause difficulties in the treatment of infections. So, extensive studies on the prevalence, genetic types, and epidemiology of CTX-M-type ESBLs are needed to refine the infection treatment.
Abstract
Background β -lactam antibiotics have been broadly used to treat gram negative microorganisms, which resulted in the occurrence of antibiotics-resistant microorganisms. Microorganisms mainly achieve resistance to antibiotics by producing β-lactamases that decrease activities of antibiotics. Various types of CTX-M extended-spectrum β -lactamases (ESBLs) have been known since CTX-M-1 and CTX-M-2 ESBLs were reported in 1996. CTX-M-14 β-lactamases were reported in Seoul and Cheju Island of Korea in 2001. Until now, there has been no CTX-M type ESBL report in Korea. So, the research on the identification of the CTX-M type ESBLs and their genetic types using E. coli resistant to or intermediate to cefotaxime were performed. Methods Total of 365 isolates of Escherichia coli from Kosin University Hospital in Busan for a six-month periods were examined. Disk diffusion test for antibiotics susceptibility, double disk synergy test for confirming ESBL, polymerase chain reaction (PCR) for identifying genetic types of CTX-M ESBLs, minimal inhibitory concentration tests of CTX-M ESBLs and isoelectricfocusing (IEF) for measuring the pi of β一lactamase were tested using E. coli resistant or intermediate to cefotaxime. Results Thirty one out of 365 E. coli isolates were resistant to or intermediate to cefotaxime. Fifteen out of 31 E. coli isolates resistant or intermediate to cefotaxime were positive to double disk synergy, 6 isolates of which were positive to PCR by CTX-M-2F and CTX-M-2R primers. MICs of cefotaxime to isolates carrying CTX-M ESBL were as high as ≥128 mg/L. MICs of ceftazidime were relatively low as 2-64 mg/L. Isoelectric point was 8.4 for blacCTX-M-3 and 8.6 for blaCTX-M-i5, respectively. Conclusion CTX-M-3 /? -lactamase and CTX-M-15 P β-lactamase found in this study are genetically different from previous types of CTX—M—type ESBLs in Korea. Also newly found CTX-M type ESBLs are expected to cause difficulties in the treatment of infections. So, extensive studies on the prevalence, genetic types, and epidemiology of CTX-M-type ESBLs are needed to refine the infection treatment.
- 발행기관:
- 고신대학교 의과대학 학술지
- 분류:
- 의학일반