LC-MS/MS를 이용한 농산물 중 제초제 fenquinotrione 및 대사산물 KIH-3653-M-2 동시시험법 개발
Development of a Simultaneous Analytical Method for Determination of Herbicide Fenquinotrione and KIH-3653-M-2 Residues in Agricultural Crops using LC-MS/MS
박지수(식품의약품안전처); 도정아(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과); 이한솔(식품의약품안전처); 조성민(식품의약품안전처); 신혜선(식품의약품안전처); 장동은(식품의약품안전처); 정용현(식품의약품안전처); 이강봉(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과)
34권 3호, 242~250쪽
초록
An analytical method was developed for the determination of fenquinotrione, a triketone herbicide, in agricultural products. Fenquinotrione was metabolized to KIH-3653-M-2 in plants. Analyte extraction was conducted using 2% formic acid in acetonitrile and cleaned up using a hydrophillic-lipophillic balance (HLB) cartridge. The limits of detection (LOD) and quantification (LOQ) were 0.004 and 0.01 mg/kg, respectively. Matrixmatched calibration curves were linear over the calibration ranges (0.001~0.1 μg/mL) into a blank extract with r 2 >0.99. The recovery results for fenquinotrione and KIH-3653-M-2 ranged between 81.1 to 116.2% and 78.0 to 110.0% at different concentration levels (LOQ, 10×LOQ, 50×LOQ) with relative standard deviation (RSD) less than 4.6%. All values were corresponded with the criteria ranges requested in both the Codex (CAC/GL 40-1993, 2003) and MFDS guidelines (2016). Therefore, the proposed method can be used as an official analytical method for determination of fenquinotrione in the Republic of Korea.
Abstract
An analytical method was developed for the determination of fenquinotrione, a triketone herbicide, in agricultural products. Fenquinotrione was metabolized to KIH-3653-M-2 in plants. Analyte extraction was conducted using 2% formic acid in acetonitrile and cleaned up using a hydrophillic-lipophillic balance (HLB) cartridge. The limits of detection (LOD) and quantification (LOQ) were 0.004 and 0.01 mg/kg, respectively. Matrixmatched calibration curves were linear over the calibration ranges (0.001~0.1 μg/mL) into a blank extract with r 2 >0.99. The recovery results for fenquinotrione and KIH-3653-M-2 ranged between 81.1 to 116.2% and 78.0 to 110.0% at different concentration levels (LOQ, 10×LOQ, 50×LOQ) with relative standard deviation (RSD) less than 4.6%. All values were corresponded with the criteria ranges requested in both the Codex (CAC/GL 40-1993, 2003) and MFDS guidelines (2016). Therefore, the proposed method can be used as an official analytical method for determination of fenquinotrione in the Republic of Korea.
- 발행기관:
- 한국식품위생안전성학회
- 분류:
- 식품위생/안전