The aqueous extract of Allium saralicum R.M. Fritsch efectively treat induced anemia: experimental study on Wistar rats
The aqueous extract of Allium saralicum R.M. Fritsch efectively treat induced anemia: experimental study on Wistar rats
Samaneh Goorani(Department of Toxicology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran); Nabi Shariatifar(Department of Environmental Health, School of Public Health , Tehran University of Medical Sciences , Tehran , Iran); Niloofar Seydi(Department of Clinical Science, Faculty of Veterinary Medicine , Razi University , Kermanshah , Iran); Akram Zangeneh(Department of Clinical Science, Faculty of Veterinary Medicine , Razi University , Kermanshah , Iran); Rohallah Moradi(Biotechnology and Medicinal Plants Research Center , Ilam University of Medical Sciences , Ilam , Iran); Behzad Tari(Department of Clinical Science, Faculty of Veterinary Medicine , Razi University , Kermanshah , Iran); Farzaneh Nazari(Department of Microbiology, Science and Research Branch , Islamic Azad University , Arak , Iran); Mohammad Mahdi Zangeneh(Department of Clinical Science, Faculty of Veterinary Medicine , Razi University , Kermanshah , Iran)
19권 4호, 403~413쪽
초록
There are many medicinal plants in traditional medicine which are used to prevent, control, and treat anemia. One of these plants is Allium saralicum R.M. Fritsch. The purpose of our research was to investigate the efect of aqueous extract of A. saralicum leaf in the treatment of hemolytic anemia. In this study, 60 rats were used. Induction of hemolytic anemia was done by three injections of Phenylhydrazine in 50 animals. Then, the rats were divided into six subgroups, including negative healthy control, untreated negative control, and four groups receiving the A. saralicum at 25, 50, 100, and 200 mg/kg concentrations. At the end of day 15 of treatment, the animals of all groups were weight and then sacrifced. The blood, liver and spleen samples were drawn immediately to analyze the hematological, biochemical and histological parameters. All groups of A. saralicum (especially AS200) signifcantly (p≤0.05) reduced the raised concentrations of Fe, ferritin, erythropoietin, ALP, AST, ALT, GGT, cholesterol, LDL, triglyceride, total and conjugated bilirubin, urea, and creatinine and increased the levels of body weight, HDL, total protein, albumin, WBC, lymphocyte, monocytes, platelet, RBC, Hb, PCV, MCV, MCH, and MCHC as compared to the untreated group. Also, A. saralicum at all doses prevented pathological changes in the liver and spleen. In conclusion, because of aqueous extract of A. saralicum leaf anti-anemic property, it can be used as a medical supplement or drug.
Abstract
There are many medicinal plants in traditional medicine which are used to prevent, control, and treat anemia. One of these plants is Allium saralicum R.M. Fritsch. The purpose of our research was to investigate the efect of aqueous extract of A. saralicum leaf in the treatment of hemolytic anemia. In this study, 60 rats were used. Induction of hemolytic anemia was done by three injections of Phenylhydrazine in 50 animals. Then, the rats were divided into six subgroups, including negative healthy control, untreated negative control, and four groups receiving the A. saralicum at 25, 50, 100, and 200 mg/kg concentrations. At the end of day 15 of treatment, the animals of all groups were weight and then sacrifced. The blood, liver and spleen samples were drawn immediately to analyze the hematological, biochemical and histological parameters. All groups of A. saralicum (especially AS200) signifcantly (p≤0.05) reduced the raised concentrations of Fe, ferritin, erythropoietin, ALP, AST, ALT, GGT, cholesterol, LDL, triglyceride, total and conjugated bilirubin, urea, and creatinine and increased the levels of body weight, HDL, total protein, albumin, WBC, lymphocyte, monocytes, platelet, RBC, Hb, PCV, MCV, MCH, and MCHC as compared to the untreated group. Also, A. saralicum at all doses prevented pathological changes in the liver and spleen. In conclusion, because of aqueous extract of A. saralicum leaf anti-anemic property, it can be used as a medical supplement or drug.
- 발행기관:
- 융합한의과학연구소
- 분류:
- 기초한의학