Evaluation of 3M™ loop-mediated isothermal amplification-based kit and 3M™ ready-to-use plating system for detection of Listeria in naturally contaminated leafy vegetables, chicken, and their related processing environments
Evaluation of 3M™ loop-mediated isothermal amplification-based kit and 3M™ ready-to-use plating system for detection of Listeria in naturally contaminated leafy vegetables, chicken, and their related processing environments
Mustapha Goni Abatcha(Universiti Sains Malaysia); Pei Ling Tan(Universiti Sains Malaysia); Li- Oon Chuah(Universiti Sains Malaysia); Gulam Rusul(Universiti Sains Malaysia); S. R. Chandraprasad(Food Safety Department, 3M Malaysia); Mohd. Esah Effarizah(Universiti Sains Malaysia)
29권 8호, 1141~1148쪽
초록
The effectiveness of two different rapid methodsinvolving the 3MTM molecular detection assay Listeria andthe 3MTM Petrifilm environmental Listeria Plate wereevaluated for the rapid detection of Listeria from naturallycontaminated vegetables and chicken-processing environmentsagainst the standard culture-based method. A total of178 samples were examined for the presence of Listeria. Atotal of 47/178 (26.4%) by standard ISO culture-basedmethod (EN ISO 11290-1), 42/178 (23.6%) by 3MTMMDA Listeria and 40/178 (22.5%) by 3MTM Petrifilm ELPlate showed positive results, respectively. The accuracy,sensitivity, specificity, positive predictive value, and negativepredictive value for 3MTM MDA Listeria and 3MTMPetrifilm EL Plate were 97.2, 89.4, 99.3, 97.7, 96.4% and96.1, 85.1, 100.0, 100.0, 94.9%, respectively. Based on theCohen’s Kappa value, there was a complete and robustconcordance between 3MTM MDA Listeria (0.911) and3MTM Petrifilm EL Plates (0.894) as compared to thestandard culture-based method.
Abstract
The effectiveness of two different rapid methodsinvolving the 3MTM molecular detection assay Listeria andthe 3MTM Petrifilm environmental Listeria Plate wereevaluated for the rapid detection of Listeria from naturallycontaminated vegetables and chicken-processing environmentsagainst the standard culture-based method. A total of178 samples were examined for the presence of Listeria. Atotal of 47/178 (26.4%) by standard ISO culture-basedmethod (EN ISO 11290-1), 42/178 (23.6%) by 3MTMMDA Listeria and 40/178 (22.5%) by 3MTM Petrifilm ELPlate showed positive results, respectively. The accuracy,sensitivity, specificity, positive predictive value, and negativepredictive value for 3MTM MDA Listeria and 3MTMPetrifilm EL Plate were 97.2, 89.4, 99.3, 97.7, 96.4% and96.1, 85.1, 100.0, 100.0, 94.9%, respectively. Based on theCohen’s Kappa value, there was a complete and robustconcordance between 3MTM MDA Listeria (0.911) and3MTM Petrifilm EL Plates (0.894) as compared to thestandard culture-based method.
- 발행기관:
- 한국식품과학회
- 분류:
- 식품과학