참나무 겨우살이와 동백나무 겨우살이 생리활성 비교
Physiological and Antimicrobial Activities of Loranthus Yadoriki and Korthalsella Japonica Ethanol Extracts
임도연(광주여자대학교)
7권 2호, 17~22쪽
초록
본 연구에서는 참나무 겨우살이와 동백나무 겨우살이 에탄올 추출물의 생리활성을 비교하여 기능성 화장품 소재로 개발하고자, 총 폴리페놀과 플라보노이드 함량, DPPH, ABTS 라디컬 소거능, SOD 유사활성, elastase, tyrosinase, α- glucosidase 저해활성을 측정하였으며 또한 RAW 264.7 세포를 이용하여 NO 생성 억제 활성을 측정하였다. 참나무 겨우살이 추출물의 총 폴리페놀과 플라보노이드 함량은 각각 98μg/mL, 54μg/mL 으로 확인 되었으며, 동백나무 겨우살이 추출물은 각각 164μg/mL, 97μg/mL 으로 확인 되었다. 500μg/mL 농도에서 DPPH 라디컬 소거능은 63.13, 80.25%, ABTS 라디컬 소거능은 75.61, 88.12%의 소거능이 확인되었다. SOD 유사활성능은 51.13, 65.52% 로 확인되었다. Tyrosinase 저해효과는 각각 58.12%, 61.13%로 확인되었으며, elastase 저해효과는 각각 32.72%, 56.71% 로 확인되었고, α- glucosidase 저해효과는 참나무 겨우살이 추출물은 61.22%, 동백나무 겨우살이 추출물은 72.51% 로 확인되었다. 항염효과를 확인하기 위해 마우스 대식세포인 RAW 264.7 세포를 이용하여 NO 저해 활성을 측정한 결과 100μg/mL 농도에서 각각 14.18, 10.98μM 까지 NO 생성량이 감소하였다. 특히, 동백나무 겨우살이 추출물이 효과가 더 높게 확인되었으며, 이러한 결과는 항산화, 미백, 항염효과를 가지는 기능성 화장품 소재로 활용 가능 할 것으로 사료된다.
Abstract
In this study, to develop a functional cosmetic material by comparing the physiological activities of oak mistletoe and camellia mistletoe extract, total polyphenol and flavonoid content, DPPH, ABTS radical scavenging activity, SOD-like activity, elastase, tyrosinase, α-glucosidase inhibition Activity was measured, and NO production inhibitory activity was also measured using RAW 264.7 cells. The total polyphenol and flavonoid contents of the oak mistletoe extract were 98μg/mL and 54μg/mL, respectively, and the camellia mistletoe extract was 164μg/mL and 97μg/mL, respectively. At a concentration of 500μg/mL, DPPH radical scavenging activity was 63.13, 80.25%, and ABTS radical scavenging activity was 75.61, 88.12%. SOD-like activity was confirmed to be 51.13, 65.52%. The tyrosinase inhibitory effect was confirmed to be 58.12% and 61.13%, respectively, and the elastase inhibitory effect was confirmed to be 32.72% and 56.71%, respectively, and the α-glucosidase inhibitory effect was confirmed to be 61.22% of oak mistletoe extract and 72.51% of camellia mistletoe extract. became In order to confirm the anti-inflammatory effect, NO inhibitory activity was measured using RAW 264.7 cells, which are mouse macrophages. As a result, the amount of NO production decreased to 14.18 and 10.98μM at a concentration of 100μg/mL, respectively. In particular, camellia mistletoe extract was confirmed to have a higher effect, and these results are considered to be available as functional cosmetic materials with antioxidant, whitening, and anti-inflammatory effects.
- 발행기관:
- 한국비즈니스학회
- 분류:
- 과학기술학