In Vitro에서 TGF β1, α-SMA 유전자 발현을 통한 Copper tripeptide의 표피재생 효과

This study aimed to investigate whether copper tripeptide, a cosmetic ingredient available on the market, has an epidermal regeneration effect in vitro. To confirm whether copper tripeptide has cytotoxicity, an MTT assay was performed, and the results showed no cytotoxicity at 100 μg/mL. Total RNA was extracted from fibroblasts (NIH3T3 cells) and epidermal keratinocytes (HaCaT cells) treated with copper tripeptide, and the mRNA expression levels of TGF β1 and α-SMA were examined using RT-PCR. The results confirmed that copper tripeptide increased the expression of TGF β1 and α-SMA genes in both epidermal keratinocytes and fibroblasts. To assess skin regeneration, a wound healing assay was used to examine cell growth and migration of NIH3T3 and HaCaT cells. The results showed that copper tripeptide had a cell regeneration-inducing effect compared to the control group in both NIH3T3 and HaCaT cells. These findings suggest that non-toxic copper tripeptide may have the potential as a cosmetic ingredient to enhance epidermal regeneration in NIH3T3 fibroblasts and HaCaT cells through increased TGF β1 and α-SMA gene expression and wound healing analysis.