Mindray MC-80 자동디지털세포형태분석기와 수동 검사법 비교: 말초혈액 도말의 비정상 세포 분석

Background: The MC-80 is a digital cell morphology analyzer developed by Mindray (China). This study evaluated the performance of the MC-80 in calculating the proportion of promonocytes and abnormal promyelocytes and compared it with manual examination. Methods: Twenty-four peripheral blood smear samples containing promonocytes and 16 samples with abnormal promyelocytes were analyzed. The MC-80 counted 200 white blood cells from the samples and calculated the proportions of blasts and blast equivalents. Subsequently, two examiners performed the same task, and the absolute reliability between the MC-80 and the examiners, as well as between the two examiners, was evaluated using the intraclass correlation coefficient (ICC). Results: For promonocyte samples, the ICC values obtained between the MC-80 and Examiners 1 and 2 were 0.772 and 0.780, respectively. For samples with abnormal promyelocytes, the ICC values for the MC-80 and Examiners 1 and 2 were 0.926 and 0.927, respectively. These values indicated “good” to “excellent” levels of absolute reliability by statistical interpretation. However, the MC-80 demonstrated lower levels of reliability with each examiner than between the two examiners, indicating a performance inferior to that of manual examination. Conclusions: The absolute reliabilities of the MC-80 and manual examination were inferior to the inter-examiner absolute reliability. However, with post-test validation, it is expected that the MC-80 could provide valuable assistance for the screening of blast equivalents.