클로렐라(Chlorella pyrenoidosa) 단백질 추출을 위한 친환경 추출법 탐색

In this study, we investigated eco-friendly physical and enzymatic methods for extracting proteins from Chlorella pyrenoidosa via cell wall decomposition. The physical methods used were hot-water extraction (HW), sonication (S), and ultrasonication (US), whereas the enzymatic methods included two cell wall-decomposing enzymes (cellulase and viscozyme L) and three proteolytic enzymes (microbial protease, papain, and bromelain) at a 1% substrate concentration. The results showed that HW and S were unsuitable for extracting chlorella proteins because of their low protein extraction yields. Conversely, among the physical treatment methods, US showed the highest protein extraction yield of 16.78±0.47%. However, physical methods can cause protein denaturation due to temperature increases with extended treatment time. In enzymatic treatment, only microbial protease proved effective, achieving the highest protein extraction yield of 36.26±2.32% after 6 h of treatment. The use of mixed enzymes did not significantly improve the yield compared to treatment with microbial protease alone. This study suggests that microbial protease is an effective method for protein extraction from chlorella and highlights its potential for application in the food industry as foundational data.