Strontium-Doped Marine Collagen Membranes Promote Osteogenesis by Inducing M2 Macrophage Polarization
Strontium-Doped Marine Collagen Membranes Promote Osteogenesis by Inducing M2 Macrophage Polarization
Xu Hao(Department of Oral Implantology, The Affiliated Hospital of Qingdao University); Li Xin(Department of Oral Implantology, The Affiliated Hospital of Qingdao University); Wang Wenxue(Department of Oral Implantology, The Affiliated Hospital of Qingdao University); Zhen Li(Department of Oral Implantology, The Affiliated Hospital of Qingdao University); Zhao Baodong(Department of Oral Implantology, The Affiliated Hospital of Qingdao University)
22권 6호, 847~864쪽
초록
Background: The design of bone biomaterials has shifted from promoting bone differentiation to “immune osteogenic coupling”. Macrophages play a key role in immune regulation, with their polarization state critically shaping the bone tissue immune microenvironment. While collagen membranes, as classic guided bone regeneration (GBR) barriers, offer excellent biocompatibility and degradability, they lack inherent bone induction and immune regulation capabilities, limiting their use in complex bone defect repair. Methods: In this study, we proposed a novel optimization strategy utilizing phase-transited lysozymes (PTL) incorporating strontium (Sr2+) into marine collagen membranes (Sr-PTL-MCM) and investigate their osteoimmunomodulatory effect through a series of experiments. Results: Sr-PTL-MCM were successfully synthesized via the PTL technique and continuously released Sr2+ ions over 7 days. Sr-PTL-MCM can effectively induce macrophage polarization from the M0 to M2 phenotype, suppresses the secretion of inflammatory cytokines, thereby enhancing mBMSCs osteogenic differentiation. RNA-sequence analysis reveals that Sr-PTL-MCM promotes M2 polarization via JAK-STAT and MAPK signaling pathways. In vivo experiments confirm its ability to create a favorable bone immune microenvironment, promoting bone growth and regeneration. Conclusion: In conclusion, incorporating Sr ions into collagen via PTL technique represents a promising approach for developing collagen membranes with immunomodulatory characteristics, thereby providing a novel and effective strategy for bone defect repair.
Abstract
Background: The design of bone biomaterials has shifted from promoting bone differentiation to “immune osteogenic coupling”. Macrophages play a key role in immune regulation, with their polarization state critically shaping the bone tissue immune microenvironment. While collagen membranes, as classic guided bone regeneration (GBR) barriers, offer excellent biocompatibility and degradability, they lack inherent bone induction and immune regulation capabilities, limiting their use in complex bone defect repair. Methods: In this study, we proposed a novel optimization strategy utilizing phase-transited lysozymes (PTL) incorporating strontium (Sr2+) into marine collagen membranes (Sr-PTL-MCM) and investigate their osteoimmunomodulatory effect through a series of experiments. Results: Sr-PTL-MCM were successfully synthesized via the PTL technique and continuously released Sr2+ ions over 7 days. Sr-PTL-MCM can effectively induce macrophage polarization from the M0 to M2 phenotype, suppresses the secretion of inflammatory cytokines, thereby enhancing mBMSCs osteogenic differentiation. RNA-sequence analysis reveals that Sr-PTL-MCM promotes M2 polarization via JAK-STAT and MAPK signaling pathways. In vivo experiments confirm its ability to create a favorable bone immune microenvironment, promoting bone growth and regeneration. Conclusion: In conclusion, incorporating Sr ions into collagen via PTL technique represents a promising approach for developing collagen membranes with immunomodulatory characteristics, thereby providing a novel and effective strategy for bone defect repair.
- 발행기관:
- 한국조직공학과 재생의학회
- 분류:
- 기타의공학