거문도닥나무(Wikstroemia ganpi) 근권 유래 Enterobacter aerogenes M167의 상추 생육촉진 효과
Growth-promoting Effect of Enterobacter aerogenes M167 isolated from the Rhizosphere of Wikstroemia ganpi on Lettuce (Lactuca sativa)
허정훈(Korea Arboreta and Gardens Institute); 손용환(National Institute of Forest Science); 안희령(Korea Arboreta and Gardens Institute); 이민성(Korea Arboreta and Gardens Institute); 임미정(Korea Arboreta and Gardens Institute); 나채선(Korea Arboreta and Gardens Institute)
68권, 492~498쪽
초록
식물생장촉진근권세균(Plant growth-promoting rhizobacteria, PGPR)은 식물의 양분 흡수를 증진하고 식물호르몬 수준을 조절하며 작물 생산성을 향상시켜 화학비료를 대체할 수 있는 친환경적 대안으로 주목받고 있다. 본 연구에서는 국내 희귀식물인 거문도닥나무(Wikstroemia ganpi)의 근권에서 분리한 Enterobacter aerogenes M167 균주의 식물생장촉진 잠재력을 평가하였다. 생리적 특성 분석 결과, M167은 인산 및 아연 가용화, 질소고정, 인돌-3-아세트산(IAA) 생성(37.35±0.57 ppm) 및 siderophore 생성(17.1±0.4 mm) 등 다기능성 생장촉진 특성을 나타냈다. 기능적 효과를 검증하기 위해 모델 작물로 상추(Lactuca sativa)에M167을 접종한 결과, 처리구는 대조구 대비 지상부 생체중이78% 증가하였다(0.89±0.58 g vs 0.50±0.24 g; Welch’s t-test, p <0.01). 반면 지상부 길이는 처리 간 유의한 차이가 없었다(대조구: 5.13±0.91 cm; M167 처리구: 5.13±1.02 cm). 이는 M167이신장 생장보다는 생체량 축적을 촉진했음을 시사한다. 종합하면, E. aerogenes M167은 상추 생육을 유의적으로 향상시키는 다기능성 PGPR로서, 자생 산림식물 근권 미생물이 지속가능 농업 및산업적 활용을 위한 유망한 생물자원임을 보여준다.
Abstract
Plant growth-promoting rhizobacteria (PGPR) enhance nutrient uptake, regulate phytohormone levels, and improve crop productivity. Thus, they can serve as eco-friendly alternative to chemical fertilizers. In the present study, we investigated the plant growth-promoting potential of Enterobacter aerogenes M167, a bacterial strain isolated from the rhizosphere of Wikstroemia ganpi, which is a threatened plant in South Korea. Physiological assays revealed multiple growth-promoting traits, including phosphate and zinc solubilization, nitrogen fixation, indole-3-acetic acid (IAA) production (37.35±0.57 ppm), and siderophore production (17.1±0.4 mm). To assess its functional effects, the M167 strain was inoculated into Lactuca sativa, which was used as the model crop. Compared with the control group, M167 treatment resulted in a 78% increase in shoot fresh weight (0.89±0.58 g vs 0.50±0.24 g; Welch’s t-test, p <0.01), while shoot length did not significantly differ between groups (control: 5.13±0.91 cm; M167: 5.13±1.02 cm). These findings suggested that M167 promoted biomass accumulation rather than elongation. Collectively, these results demonstrate that M167 is a multifunctional PGPR that significantly enhances lettuce growth, underscoring the potential of rhizosphere microorganisms found in native forest plants as promising bioresources for sustainable agriculture and industrial applications.
- 발행기관:
- 한국응용생명화학회
- 분류:
- 농화학