RNA interference with the selenoprotein M gene inhibits the growth promoting effect by selenium supplementation to silkworm Bombyx mori
RNA interference with the selenoprotein M gene inhibits the growth promoting effect by selenium supplementation to silkworm Bombyx mori
Ma Run-Hui(North Minzu University); Ge Wen-Jie(Hefei University of Technology); Hu Fei(School of Food and Biological Engineering, Hefei University of Technology); Hong Ting-Ting(School of Food and Biological Engineering, Hefei University of Technology); Thakur Kiran(School of Food and Biological Engineering, Hefei University of Technology); Tang Shunming(Jiangsu University of Science and Technology); Wei Zhao-Jun.(North Minzu University)
29권 1호, 1~11쪽
초록
Moderate selenium (Se) supplementation has been shown to promote silkworm growth; however, its metabolic pathway in insects remains poorly understood. In organisms, Se is primarily incorporated as selenocysteine into selenoproteins, including selenoprotein M (SelM), a thioredoxin fold oxidoreductase highly expressed in brain tissue that modulates cellular redox homeostasis and confers neuroprotection. Herein, we predicted the physi cochemical and structural features of the silkworm SelM. Gene-specific siRNAs were then micro-injected into silkworms fed with sodium selenite (Na 2 SeO 3 ) (50 μM). SelM disruption reduced body mass, cocoon weight, cocoon shell weight and shell rate of silkworms, whereas egg production and egg laying mostly remained un changed. Moreover, sodium selenite (Na 2 SeO 3 ) (50 μM) supplementation alone significantly improved growth factors of silkworms, without any obvious effect on egg production and laying. RT-qPCR revealed lowest SelM transcript levels at 24 h post injection, followed by a gradual recovery; among the three siRNAs tested, SelM-244 had the strongest interfering effect. These data demonstrate that SelM is an indispensable component of Se metabolism in silkworms, coupling Se status to silk protein synthesis and larval growth. Targeted knockdown of SelM provides a tractable platform for dissecting Se metabolism in insects and for designing Se-biofortified sericulture.
Abstract
Moderate selenium (Se) supplementation has been shown to promote silkworm growth; however, its metabolic pathway in insects remains poorly understood. In organisms, Se is primarily incorporated as selenocysteine into selenoproteins, including selenoprotein M (SelM), a thioredoxin fold oxidoreductase highly expressed in brain tissue that modulates cellular redox homeostasis and confers neuroprotection. Herein, we predicted the physi cochemical and structural features of the silkworm SelM. Gene-specific siRNAs were then micro-injected into silkworms fed with sodium selenite (Na 2 SeO 3 ) (50 μM). SelM disruption reduced body mass, cocoon weight, cocoon shell weight and shell rate of silkworms, whereas egg production and egg laying mostly remained un changed. Moreover, sodium selenite (Na 2 SeO 3 ) (50 μM) supplementation alone significantly improved growth factors of silkworms, without any obvious effect on egg production and laying. RT-qPCR revealed lowest SelM transcript levels at 24 h post injection, followed by a gradual recovery; among the three siRNAs tested, SelM-244 had the strongest interfering effect. These data demonstrate that SelM is an indispensable component of Se metabolism in silkworms, coupling Se status to silk protein synthesis and larval growth. Targeted knockdown of SelM provides a tractable platform for dissecting Se metabolism in insects and for designing Se-biofortified sericulture.
- 발행기관:
- 한국응용곤충학회
- 분류:
- 기타농생물